Vertical Transmission of Salmonella enterica ser. Gallinarum in Dermanyssus gallinae by the Mean of the Baudruche-Based Artificial Feeding Device

The poultry red mite (PRM) Dermanyssus gallinae is well known for its vectorial role pathogens, such as Salmonella enterica ser. Gallinarum, the causative agent of fowl typhoid. Here, we ascertained vertical transmission S. Gallinarum across PRM life stages, combining Baudruche-based in vitro feeding system and a PRM-fitting DNA extraction detection method by qPCR. Small-sized pools (4–5 specimens) adult mites, eggs, larvae, protonymphs, single were tested Gallinarum. pathogen was detected 89% 5% 17% pooled 9% 43% protonymphs. Additionally, rate infected uninfected mites similar, while differences ovipositing fecundity observed. allowed to confirm infection through bloodmeal strongly suggest stages. Furthermore, it allows avoid vivo studies could be useful further investigating D. or other hematophagous arthropods infectious agents.